High performance liquid chromatography of natural products. I. Separation of cephalosporin C derivatives and cephalosporin antibiotics; isolation of cephalosporin C from fermentation broth.
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چکیده
منابع مشابه
Biosynthesis of Cephalosporin C: I. Factors Affecting the Fermentation.
A series of complex and synthetic media have been developed that are suitable for the production of cephalosporin C and cephalosporin N by a mutant strain of Cephalosporium (C.M.I. 49,137). dl-Methionine increased the yield of both antibiotics, with more effect on cephalosporin N. l-Cystine had a greater enhancing effect on formation of cephalosporin C than on formation of cephalosporin N in sy...
متن کاملBiosynthesis of cephalosporin C.
The superiority of d-methionine over l-methionine for stimulation of cephalosporin C synthesis in a crude medium was confirmed. The optimal level of dl-methionine was 0.5%. Methionine stimulates growth slightly but this is not thought to be the cause of the marked stimulation of antibiotic synthesis. Of a large number of sulfur compounds tested, only dl-methionine-dl-sulfoxide and S-methyl-l-cy...
متن کاملProcess strategies for cephalosporin C fermentation
Cephalosporin C (CPC), a β-lactam antibiotic, is starting molecule for industrial production of semi synthetic cephalosporins. CPC fermentation has been carried out in Air Lift Reactor (indigenously made up of borosilicate glass) in different fermentation modes i.e., batch and fed batch modes using a mold Acremonium chrysogenum (synn. Cephalosporium acremonium). A definite correlation exists be...
متن کاملFormation of desacetylcephalosporin C in cephalosporin C fermentation.
The origin of desacetylcephalosporin C in cephalosporin C fermentation broths was investigated. Esterase activity was detected in cell-free extracts of Cephalosporium acremonium, but these extracts failed to deesterify cephalosporin C. When cephalosporin C was added to sterile and inoculated fermentation media, the antibiotic decayed at nearly identical rates. The formation of desacetylcephalos...
متن کاملPharmacokinetics of cephalosporin antibiotics.
Vol. 23: Fundamentals in Cancer Chemotherapy. Anticancer Symposium held in Connection with the 10th International Congress of Chemotherapy, Zurich, September 1977. Ed.: F. M. SCHABEL, Jr. (Birmingham, Ala.). VIII +3 04 p., 62 fig., 1 cpl., 91 tab., 1978 ISBN 3-8055-2830-2 Vol. 24: Application of Cancer Chemotherapy. Anticancer Symposium held in Connection with the 10th International Congress of...
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ژورنال
عنوان ژورنال: The Journal of Antibiotics
سال: 1976
ISSN: 0021-8820,1881-1469
DOI: 10.7164/antibiotics.29.902